大三巴牌坊（葡萄牙語：Ruínas de São Paulo）是澳門天主之母教堂（即聖保祿教堂）正面前壁的遺址，屬於「聖保祿學院天主之母教堂遺址（大三巴牌坊、前地及石階）」的組成部分之一，亦是澳門的標誌性建築物之一。「大三巴」一名為華人對聖保祿學院及教堂的稱呼，出自聖保祿的葡萄牙文「São Paulo」的漢語譯音。
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